DNA Oligo Synthesis and Primers
AltaBioscience has been supplying custom DNA oligos and primers of outstanding quality for various applications in a ready-to-use form for 50 years. Our DNA oligo synthesis service offers a full range of modifications, scales, purification options and fluorescently tagged custom DNA primers.
Here at AltaBioscience, our DNA oligo synthesis service offers single-stranded DNA (ssDNA) with a fast turnaround from our ISO 9001-certified laboratory.
*Please note that these are starting scales. Your final product may contain less than that amount due to synthesis and purification losses.
Applications for DNA oligo synthesis and DNA primers
DNA oligos and DNA primers are critical components of a wide range of molecular biology applications, from basic research to clinical diagnostics. Oligos are used routinely for:
- PCR primers
- DNA sequencing and barcoding
- Conjugation to a peptide carrier, e.g., to transport a peptide inside the cell nucleus
- Gene detection, synthesis and cloning
- Site-directed mutagenesis
- Fluorescence in situ hybridization (FISH)
- Antisense oligonucleotide therapy
- RNA interference (RNAi)
- Aptamer development
- Microarray analysis
- CRISPR/Cas9 genome editing
- Detection and identification of genetic mutations or polymorphisms
- SNP genotyping (Single Nucleotide Polymorphism)
Custom DNA oligo synthesis method
DNA oligos are synthesised using solid-phase methods. The synthesis is performed using automated synthesisers where reactions proceed rapidly, one base addition occurring every four minutes with very high efficiencies. The chemistry is well established with the base at the 3’ of the target oligo being attached to a controlled pore glass support, with the oligo grown automatically base by base towards the 5’ end.
Degenerate oligos (mixed bases) of any combination can be added at any position at no extra cost. Codes for degenerate bases are as follows:
Unusual bases
Internal modified bases, e.g. Inosine, Uracil, Oxo dG, Bromo dU and Hydroxy dC, can be added anywhere along the chain.
3’ and 5’ modifications
These include groups such as biotin, amino, thiol, phosphate groups or linkers. Incorporation of dyes and quenchers at the 3’ end is available, with options such as BHQ 1, BHQ 2, BHQ 3, Dabcyl and FAM readily available.
Frequently asked questions
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High-quality Peptide-Oligonucleotide Conjugates for Life Science research
We are pleased to provide custom production of peptide-oligonucleotide conjugates (POCs) with industry-leading turnaround times.
DNA Peptide Conjugates
At AltaBioscience, we offer different options for conjugation either at the 3’ end of the oligo or at the 5’ prime end, depending on your requirements. In addition, the linkage between the DNA-peptide can be either a stable covalent bond or a reducible disulphide bridge as needed for your application.